Metadata details for analysis AN004460
Study IDST002749
Analysis IDAN004460
Study TitleBromeliad lipidomics for adaptation to elevation
InstituteUniversity of Campinas
SpeciesPitcairnia flammea
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Scientific UltiMate™ 3000 UHPLC RSLCnano system
Chromatography TypeReversed phase
Chromatography ColumnSupelco Sigma-Aldrich Titan C18 (100 x 2.1mm, 1.9um)
Solvent A40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B10% acetonitrile/90% isopropanol; 10 mM ammonium acetate
Gradient250 µL/min: 0-2 min: 40% B, 2-3 min: 50% B, 3-6 min: 50% B, 6.1-8 min: 70% B, 8-9 min: 100% B, 9-11 min: 100% B, 11-12 min: 40% B, 12-14 min: 40% B.
Flow rate250 µL/min
Column Temperature40
Retention time unitsMinutes
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