Metadata details for analysis AN004501 | |
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Study ID | ST002765 |
Analysis ID | AN004501 |
Study Title | Disorder of Lipids Induced by Gestational Asthma and its Effect on the Development of Fetal Lung Function and Fetal Health |
Institute | Nanjing University of Chinese Medicine |
Species | Rattus norvegicus |
Ion_mode | NEGATIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive Orbitrap |
MS Instrument Type | Other |
Chromatography Instrument Name | Thermo UltiMate 3000 RS |
Chromatography Type | Reversed phase |
Chromatography Column | Waters Acquity UPLC CSH C18 column (100 mm×2.1mm, 1.7 m) |
Solvent A | 60%acetonitrile;40%water |
Solvent B | 90%isopropanol;10% acetonitrile;0.1% formic acid;10mM ammonium formate |
Gradient | 15% B at 0 min, 30% B at 0–2 min, 48% B at 2–2.5 min, 82% B at 2.5–11 min, 99% B at 11–12 min and 15% B at 12–15 min. |
Flow rate | 0.6 mL/min |
Column Temperature | 65℃ |
Retention time units | Minutes |