Metadata details for analysis AN004504
Study IDST002768
Analysis IDAN004504
Study TitleDysregulation of neural activity and microglia function following exposure to the global environmental contaminant perfluorooctane sulfonate (PFOS)
InstituteBrown University
SpeciesDanio rerio
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF-X Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Vanquish
Chromatography TypeReversed phase
Chromatography ColumnThermo Hypersil Gold Vanquish, 50 mm X 2.1 mm x 1.9 µm
Solvent A2 mM aqueous ammonium acetate
Solvent B2 mM ammonium acetate in acetonitrile
Gradientequilibration with 2.5% B for 1 minute, increase to 100% B over 11 minutes and held for 2 minutes, and back to 2.5% B over 1 minute and held for 1.5 minutes (total run time 16.5 minutes, data were collected from 0.05 to 12.5 minutes)
Flow rate0.5 mL/minute
Column Temperature60ºC
Retention time unitsSeconds
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