| Metadata details for analysis AN004504 | |
|---|---|
| Study ID | ST002768 |
| Analysis ID | AN004504 |
| Study Title | Dysregulation of neural activity and microglia function following exposure to the global environmental contaminant perfluorooctane sulfonate (PFOS) |
| Institute | Brown University |
| Species | Danio rerio |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF-X Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Thermo Hypersil Gold Vanquish, 50 mm X 2.1 mm x 1.9 µm |
| Solvent A | 2 mM aqueous ammonium acetate |
| Solvent B | 2 mM ammonium acetate in acetonitrile |
| Gradient | equilibration with 2.5% B for 1 minute, increase to 100% B over 11 minutes and held for 2 minutes, and back to 2.5% B over 1 minute and held for 1.5 minutes (total run time 16.5 minutes, data were collected from 0.05 to 12.5 minutes) |
| Flow rate | 0.5 mL/minute |
| Column Temperature | 60ºC |
| Retention time units | Seconds |
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