| Metadata details for analysis AN004505 | |
|---|---|
| Study ID | ST002768 |
| Analysis ID | AN004505 |
| Study Title | Dysregulation of neural activity and microglia function following exposure to the global environmental contaminant perfluorooctane sulfonate (PFOS) |
| Institute | Brown University |
| Species | Danio rerio |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF-X Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | Thermo Syncronis HILIC 50 mm X 2.1 mm x 3 µm |
| Solvent A | 2 mM ammonium acetate in acetonitrile |
| Solvent B | 2 mM aqueous ammonium acetate |
| Gradient | equilibrate with 10% B for 1 minute, increase to 65% B for 9 minutes and hold for 3 minutes, decrease to 10% over 1 minute and hold for 1 minute |
| Flow rate | 0.2 mL/minute |
| Column Temperature | 25ºC |
| Retention time units | Seconds |