| Metadata details for analysis AN004514 | |
|---|---|
| Study ID | ST002773 |
| Analysis ID | AN004514 |
| Study Title | A nested case-control study of untargeted plasma metabolomics and lung cancer risk among never-smoking women in Shanghai Women’s Health Study |
| Institute | Emory University |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 RS |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters XBridge BEH Amide (50 x 2.1mm,2.5um) |
| Solvent A | 100% water |
| Solvent B | 100% acetonitrile |
| Gradient | Initial mobile phase conditions are 60% A, 35% B, 5% C hold for 0.5 min, with linear gradient to 0% A, 95% B, 5% C at 1.5 min, hold for 3.5 min, resulting in a total analytical run time of 5 min. During the flushing phase (HILIC analytical separation), the C18 column is equilibrated with a wash solution of 0% A, 95% B, 5% C until 2.5 min, followed by an equilibration solution of 60% A, 35% B, 5% C for 2.5 min. |
| Flow rate | 0.4-0.5 mL/min |
| Column Temperature | 40 |
| Retention time units | Seconds |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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