Metadata details for analysis AN004537
Study IDST002788
Analysis IDAN004537
Study TitleMetabolomic analysis of particulate matter in the NPSG during a 2018 cruise on the R/V Falkor
InstituteUniversity of Washington
SpeciesNatural mixed marine microbial community
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF hybrid Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameWaters Acquity I-Class
Chromatography TypeHILIC
Chromatography ColumnSeQuant ZIC-HILIC (150 x 2.1mm, 5um)
Solvent A85% acetonitrile/15% water
Solvent B15% acetonitrile/85% water
Gradient100% A for 2 minutes, ramped to 64% B over 18 minutes, ramped to 100% B over 1 minute, held at 100% B for 5 minutes, and equilibrated at 100% A for 25 minutes (50 minutes total)
Flow rate0.15 mL/min
Column Temperature30
Retention time unitsMinutes
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