Metadata details for analysis AN004540 | |
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Study ID | ST002790 |
Analysis ID | AN004540 |
Study Title | Community metabolomes reflect taxon-specific fingerprints of phytoplankton and heterotrophic bacteria in the ocean (expanded) |
Institute | University of Washington |
Species | Marine plankton |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Waters Acquity I-Class |
Chromatography Type | HILIC |
Chromatography Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
Solvent A | 85% acetonitrile/15% water; 10 mM ammonium carbonate |
Solvent B | 15% acetonitrile/85% water; 10 mM ammonium carbonate |
Gradient | 100% A for 2 minutes, ramped to 64% B over 18 minutes, ramped to 100% B over 1 minute, held at 100% B for 5 minutes, and equilibrated at 100% A for 25 minutes (50 minutes total) |
Flow rate | 0.15mL/min |
Column Temperature | 30 |
Retention time units | Minutes |