| Metadata details for analysis AN004555 | |
|---|---|
| Study ID | ST002799 |
| Analysis ID | AN004555 |
| Study Title | Gestational asthma causes lipid disturbance in fetal alveolar lavage fluid |
| Institute | Nanjing University of Chinese Medicine |
| Species | Rattus norvegicus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Other |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 RS |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Solvent A | 60％acetonitrile；40％water |
| Solvent B | 90％isopropanol；10％ acetonitrile；0.1% formic acid；10mM ammonium formate |
| Gradient | 15% B at 0 min, 30% B at 0–2 min, 48% B at 2–2.5 min, 82% B at 2.5–11 min, 99% B at 11–12 min and 15% B at 12–15 min. |
| Flow rate | 0.6 mL/min |
| Column Temperature | 65℃ |
| Retention time units | Minutes |
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