| Metadata details for analysis AN004558 | |
|---|---|
| Study ID | ST002802 |
| Analysis ID | AN004558 |
| Study Title | Nontargeted Serum Metabolomic Profiling of FXR-null and Wild-type Mice |
| Institute | University of North Carolina at Chapel Hill |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | 2% B from 0-1 min; 2%-15% B from 1-3 min; 15%-50% B from 3-6 min; 50%-98% B from 6-7.5 min; 98% B held from 7.5-11.5 min; 98%-2% B from 11.5-11.6 min; and 2% B held from 11.6-15 min for a final re-equilibration. |
| Flow rate | 0.4 mL/min |
| Column Temperature | 40 |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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