| Metadata details for analysis AN004562 | |
|---|---|
| Study ID | ST002806 |
| Analysis ID | AN004562 |
| Study Title | Comprehensive Metabolic Profiling of MYC-Amplified Medulloblastoma Tumors Reveals Key Dependencies on Amino Acid, Tricarboxylic Acid and Hexosamine Pathways |
| Institute | Johns Hopkins University |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6520 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 1290 |
| Chromatography Type | HILIC |
| Chromatography Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | 0.3 mL/minute. Mobile phases consisted of A (water + 0.1% formic acid) and B (acetonitrile + 0.1% formic acid). The column was equilibrated at 2.5/97.5 (A/B) and maintained for 1 min post injection. Mobile-phase A increased in a linear gradient from 2.5% to 65% from 1 to 9 min post injection then stepped to 97.5% A from 9 to 11 min to wash the column. |
| Flow rate | 0.3 mL/minute |
| Column Temperature | 45 |
| Retention time units | Minutes |
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