Metadata details for analysis AN004562
Study IDST002806
Analysis IDAN004562
Study TitleComprehensive Metabolic Profiling of MYC-Amplified Medulloblastoma Tumors Reveals Key Dependencies on Amino Acid, Tricarboxylic Acid and Hexosamine Pathways
InstituteJohns Hopkins University
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameAgilent 6520 QTOF
MS Instrument TypeQTOF
Chromatography Instrument NameAgilent 1290
Chromatography TypeHILIC
Chromatography ColumnWaters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Solvent A100% water; 0.1% formic acid
Solvent B100% acetonitrile; 0.1% formic acid
Gradient0.3 mL/minute. Mobile phases consisted of A (water + 0.1% formic acid) and B (acetonitrile + 0.1% formic acid). The column was equilibrated at 2.5/97.5 (A/B) and maintained for 1 min post injection. Mobile-phase A increased in a linear gradient from 2.5% to 65% from 1 to 9 min post injection then stepped to 97.5% A from 9 to 11 min to wash the column.
Flow rate0.3 mL/minute
Column Temperature45
Retention time unitsMinutes
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