| Metadata details for analysis AN004611 | |
|---|---|
| Study ID | ST002826 |
| Analysis ID | AN004611 |
| Study Title | Cold-stimulated brown adipose tissue activation is related to changes in serum metabolites relevant to NAD+ metabolism in humans |
| Institute | University of Turku |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6540 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 1290 |
| Chromatography Type | HILIC |
| Chromatography Column | Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm |
| Solvent A | 50% acetonitrile with 20 mM ammonium formate and 0.25% formic acid, pH 3 |
| Solvent B | 90% acetonitrile with 20 mM ammonium formate, 0.25% formic acid, pH 3. |
| Gradient | 0–2.5 min: 100% B, 2.5–10 min: 100% B → 0% B; 10–10.01 min: 0% B → 100% B; 10.01–12.5 min: 100% B. |
| Flow rate | 0.6 mL/min. |
| Column Temperature | 45 |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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