| Metadata details for analysis AN004630 | |
|---|---|
| Study ID | ST002834 |
| Analysis ID | AN004630 |
| Study Title | Resource competition predicts assembly of in vitro gut bacterial communities- 2021-C18 |
| Institute | Stanford University |
| Species | Bacteroides thetaiotaomicron |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent Zorbax SB-C18 (100 x 3.0 mm, 1.8 um) |
| Solvent A | Water + 0.1% formic acid |
| Solvent B | Acetonitrile + 0.1% formic acid |
| Gradient | Gradient elution was performed from 100% (B) at 0–2 min to 70% (B) at 7.7 min, 40% (B) at 9.5 min, 30% (B) at 10.25 min, 100% (B) at 12.75 min, isocratic until 16.75 min with a column flow ofGradient elution was performed as follows 3% (B) maintained 0–0.43 min to 97% (B) at 9 min, maintained until 11 min, returning to initial conditions at 11.5 min and equilibrating until the end of the run at 14 min. |
| Flow rate | 0.4 mL/min. |
| Column Temperature | 40C |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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