Metadata details for analysis AN004663
Study IDST002845
Analysis IDAN004663
Study TitleMethylprednisolone therapy induces differential metabolic trajectories in severe COVID-19 patients
InstituteFederal University of Goiás
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameAgilent 1220 Infinity
Chromatography TypeReversed phase
Chromatography ColumnAgilent Zorbax Eclipse Plus C18 (150 x 4.6mm,3.5um)
Solvent A100% water; 0.5% formic acid; 5 mM of ammonium formate
Solvent B100% acetonitrile
Gradientgradient elution started with 20% (B) for 5 min, then linearly increased to 100% (B) in 30 min and kept constant for 8 min in 100% (B). The eluent was restored to the initial conditions in 4 minutes to re-equilibrate the column and held for the remaining 8 minutes.
Flow rate0.5 mL/min
Column Temperature35
Retention time unitsMinutes
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