Metadata details for analysis AN004666
Study IDST002848
Analysis IDAN004666
Study TitleLipidomics for linked CD1 proteins
InstituteBrigham and Women's Hospital
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameAgilent 6520 QTOF
MS Instrument TypeQTOF
Chromatography Instrument NameAgilent 1200
Chromatography TypeNormal phase
Chromatography ColumnFirst through a MetaGuard guard column (2 mm, 3 μm, Varian, A0542-MG2) and then a MonoChrom Diol column (150 X 2 mm, 3μm, Varian, A0542150X020)
Solvent A70% isopropanol, 30% methanol, 0.1% formic acid, and 0.05% ammonium hydroxide
Solvent B70% hexanes, 30% isopropanol, 0.1% formic acid, and 0.05% ammonium hydroxide
GradientThe binary gradient was monitored with solvent B with 100% at 0-10 min, 50% at 17-22 min, 0% at 30-35 min, and 100% at 40-50 min, followed by an additional 0% for 6 min post-run for regeneration.
Flow rate0.7 ml/min
Column Temperature22
Retention time unitsMinutes
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