Metadata details for analysis AN004675
Study IDST002854
Analysis IDAN004675
Study TitleHILIC-IM-MS for Simultaneous Lipid and Metabolite Profiling of Microorganisms
InstituteUniversity of Georgia
SpeciesStaphylococcus aureus/ Acinetobacter baumannii/ Enterococcus faecium/ Pseudomonas aeruginosa
Ion_modePOSITIVE
MS typeESI
MS Instrument NameWaters Synapt G2 XS
MS Instrument TypeQTOF
Chromatography Instrument NameWaters Acquity
Chromatography TypeHILIC
Chromatography ColumnWaters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Solvent A100% water; 10 mM ammonium formate; 0.125% formic acid
Solvent B95% ACN/5% water; 10 mM ammonium formate; 0.125% formic acid
Gradient0-2 min at 100% B, 2-7.7 min from 100% to 70% B, 7.7-9.5 min from 70% to 40% B, 9.5-10.25 min from 40% to 30% B, 10.25-12.75 min from 30% to 100% B, and 12.75-17 min to re-equilibrate to 100% B
Flow rate0.4 mL/min
Column Temperature45
Retention time unitsMinutes
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