| Metadata details for analysis AN004676 | |
|---|---|
| Study ID | ST002854 |
| Analysis ID | AN004676 |
| Study Title | HILIC-IM-MS for Simultaneous Lipid and Metabolite Profiling of Microorganisms |
| Institute | University of Georgia |
| Species | Staphylococcus aureus/ Acinetobacter baumannii/ Enterococcus faecium/ Pseudomonas aeruginosa |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Waters Synapt G2 XS |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Waters Acquity |
| Chromatography Type | HILIC |
| Chromatography Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| Solvent A | 100% water; 10 mM ammonium formate; 0.125% formic acid |
| Solvent B | 95% ACN/5% water; 10 mM ammonium formate; 0.125% formic acid |
| Gradient | 0-2 min at 100% B, 2-7.7 min from 100% to 70% B, 7.7-9.5 min from 70% to 40% B, 9.5-10.25 min from 40% to 30% B, 10.25-12.75 min from 30% to 100% B, and 12.75-17 min to re-equilibrate to 100% B |
| Flow rate | 0.4 mL/min |
| Column Temperature | 45 |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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