Metadata details for analysis AN004803 | |
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Study ID | ST002929 |
Analysis ID | AN004803 |
Study Title | Hypoxia-inducible factor-1a (HIF1a) physiology in mouse skeletal muscle |
Institute | Cleveland Clinic |
Species | Mus musculus |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive Focus |
MS Instrument Type | QTRAP |
Chromatography Instrument Name | Thermo Vanquish |
Chromatography Type | HILIC |
Chromatography Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
Solvent A | 100% water;10 mM ammonium acetate; 0.125% acetic acid |
Solvent B | 5% water/95% acetonitrile;10 mM ammonium acetate; 0.125% acetic acid |
Gradient | Time (min.) Flow Rate (mL/min) %B 0.0 0.2 100 2.0 0.2 70 4.0 0.2 60 5.5 0.2 60 10.5 0.2 50 11.5 0.2 50 12.0 0.2 100 16.0 0.2 100 |
Flow rate | 0.2 mL/min |
Column Temperature | 40 |
Retention time units | Seconds |