Metadata details for analysis AN004805 | |
---|---|
Study ID | ST002929 |
Analysis ID | AN004805 |
Study Title | Hypoxia-inducible factor-1a (HIF1a) physiology in mouse skeletal muscle |
Institute | Cleveland Clinic |
Species | Mus musculus |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive Focus |
MS Instrument Type | QTRAP |
Chromatography Instrument Name | Thermo Vanquish |
Chromatography Type | Reversed phase |
Chromatography Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
Solvent A | 100% water; 0.1% formic acid |
Solvent B | 100% acetonitrile; 0.1% formic acid |
Gradient | Time (min.) Flow Rate (mL/min) %B 0.2 0.3 5 1.0 0.3 15 8.0 0.3 95 10.0 0.3 95 12.0 0.3 5 15.0 0.3 5 |
Flow rate | 0.3 mL/min |
Column Temperature | 60 |
Retention time units | Seconds |