| Metadata details for analysis AN004810 | |
|---|---|
| Study ID | ST002932 |
| Analysis ID | AN004810 |
| Study Title | Analyzing Metabolic Alterations in the Gut, Hippocampus and Brainstem of Mice Induced by Running Exercise Through Liquid Chromatography Mass Spectrometry |
| Institute | University of Puerto Rico, School of Medicine |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Waters Synapt-XS |
| MS Instrument Type | Ion Mobility TOF |
| Chromatography Instrument Name | Synapt-XS Waters |
| Chromatography Type | HILIC |
| Chromatography Column | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | 5% to 50% A was applied over 8 minutes, followed by a 2-minute hold at 50% B before returning to the initial condition for a 5-minute re-equilibration. |
| Flow rate | 0.4 mL/min |
| Column Temperature | 50ºC |
| Retention time units | Minutes |
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