| Metadata details for analysis AN004861 | |
|---|---|
| Study ID | ST002960 |
| Analysis ID | AN004861 |
| Study Title | Analysis of Lipids Secreted from Fibroblast Young Cells |
| Institute | Ajou University Medical Center |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Agilent 1290 Infinity UHPLC |
| Chromatography Type | Reversed phase |
| Chromatography Column | Thermo Hypersil GOLD C18 (100 x 2.1mm,1.9um) |
| Solvent A | Acetonitrile:Methanol:Water (19:19:2, v/v/v) + 20 mmol/L ammonium formate + 0.1% formic acid (v/v) |
| Solvent B | 2-propanol + 20 mmol/L ammonium formate + 0.1% formic acid (v/v) |
| Gradient | 0–5 min, 5% B; 5–15 min, 5–30% B; 15–22 min, 30–90% B; 22–25 min, 90% B; 25–26 min, 90–5% B; 26–30 min, 5% B. Parameters were set as follows: positive mode, spray voltage; 3.8 kV |
| Flow rate | 0.2 mL/min |
| Column Temperature | 320℃ |
| Retention time units | Minutes |
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