Metadata details for analysis AN004861
Study IDST002960
Analysis IDAN004861
Study TitleAnalysis of Lipids Secreted from Fibroblast Young Cells
InstituteAjou University Medical Center
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive Hybrid Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameAgilent 1290 Infinity UHPLC
Chromatography TypeReversed phase
Chromatography ColumnThermo Hypersil GOLD C18 (100 x 2.1mm,1.9um)
Solvent AAcetonitrile:Methanol:Water (19:19:2, v/v/v) + 20 mmol/L ammonium formate + 0.1% formic acid (v/v)
Solvent B2-propanol + 20 mmol/L ammonium formate + 0.1% formic acid (v/v)
Gradient0–5 min, 5% B; 5–15 min, 5–30% B; 15–22 min, 30–90% B; 22–25 min, 90% B; 25–26 min, 90–5% B; 26–30 min, 5% B. Parameters were set as follows: positive mode, spray voltage; 3.8 kV
Flow rate0.2 mL/min
Column Temperature320℃
Retention time unitsMinutes
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