| Metadata details for analysis AN004862 | |
|---|---|
| Study ID | ST002961 |
| Analysis ID | AN004862 |
| Study Title | Analysis of Metabolites Secreted from Fibroblast Young Cells |
| Institute | Ajou University Medical Center |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Agilent 1290 Infinity UHPLC |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm, 1.8um) |
| Solvent A | 0.1% formic acid in water |
| Solvent B | 0.1% formic acid in 80% acetonitrile |
| Gradient | 2.5% solvent B in 5 min, 2.5–12.5% solvent B in 29 min, 12.5–25% solvent B in 11 min, 25–37.5% solvent B in 11 min, 37.5-80% solvent B in 0.1 min, holding at 80% of solvent B in 13.9 min, 80–2.5% solvent B in 0.1 min, 2.5% solvent B for 19.9 min |
| Flow rate | 0.2 mL/min |
| Column Temperature | 320℃ |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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