| Metadata details for analysis AN004866 | |
|---|---|
| Study ID | ST002964 |
| Analysis ID | AN004866 |
| Study Title | Comprehensive Untargeted LC-MS Metabolomics Analysis of CYP19A1/PRLR/PTN Knockout iPSCs |
| Institute | The Jackson Laboratory for Genomic Medicine |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF-X Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| Solvent A | 95% ACN/water (10 mM ammonium acetate, 0.1% acetic acid) |
| Solvent B | 50% ACN/water (10 mM ammonium acetate, 0.1% acetic acid) |
| Gradient | From 0 to 0.20 minutes, B is 0% with a flow rate of 550 µL/min; it jumps to 98% by 8.75 minutes (still at 550 µL/min) and remains until 10 minutes; at 15 minutes, B returns to 0% but the flow drops to 100 µL/min, then rises back to 550 µL/min by 17 minutes, staying consistent until 20 minutes. |
| Flow rate | 550->100->550µL/min |
| Column Temperature | 45 |
| Retention time units | Seconds |
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