Metadata details for analysis AN004882
Study IDST002973
Analysis IDAN004882
Study TitleExamine the through-filter recovery of metabolites extracted from a complex bacterial medium
InstituteDuke University
Species
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Orbitrap Exploris 240
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Vanquish
Chromatography TypeReversed phase
Chromatography ColumnWaters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Solvent A100% water + 0.1% formic acid
Solvent B100% methanol + 0.1% formic acid
GradientFrom 0.5% B to 70% B in 4 minutes, from 70% B to 98% B in 0.5 minutes, and holding at 98% B for 0.9 minute before returning to 0.5% B in 0.2 minutes.
Flow rate0.350 mL/minute
Column Temperature40C
Retention time unitsMinutes
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