Metadata details for analysis AN004897
Study IDST002980
Analysis IDAN004897
Study TitleWater soluble metabolomics in mice upon loss of SHMT
InstitutePrinceton University
SpeciesMus musculus
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive Plus Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Vanquish
Chromatography TypeHILIC
Chromatography ColumnWaters XBridge BEH Amide (100 x 2.1mm,2.5um)
Solvent A95% water/5% acetonitrile with 20 mM ammonium acetate, 20 mM ammonium hydroxide, pH 9.4
Solvent B100% acetonitrile
Gradient0 minutes, 85% B; 2 minutes, 85% B; 3 minutes, 80% B; 5 minutes, 80% B; 6 minutes, 75% B; 7 minutes, 75% B; 8 minutes, 70% B; 9 minutes, 70% B; 10 minutes, 50% B; 12 minutes, 50% B; 13 minutes, 25% B; 16 minutes, 25% B; 18 minutes, 0% B; 23 minutes, 0% B; 24 minutes, 85% B; 30 minutes, 85% B
Flow rate150 μl/min
Column Temperature25°C
Retention time unitsMinutes
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