| Metadata details for analysis AN004899 | |
|---|---|
| Study ID | ST002981 |
| Analysis ID | AN004899 |
| Study Title | Developmental Neurotoxicity of Deltamethrin Exposure on Hypothalamic Neurogenesis in Embryonic Zebrafish |
| Institute | College of Marine Food and Biological Engineering, Jimei University |
| Species | Danio rerio |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Orbitrap Exploris 120 |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Solvent A | Acetonitrile/water (6:4, v/v) |
| Solvent B | Isopropanol/acetonitrile (9:1, v/v) |
| Gradient | First maintain 32% B for 1.5 minutes, then linearly increase from 1.5 to 15.5 minutes to 85% B, then linearly increase to 97% B at 0.1 minutes, maintain for 18 minutes, and then rapidly decrease to 32% B at 0.1 minutes, equilibrate until the next injection |
| Flow rate | 0.4 mL/min |
| Column Temperature | 55 |
| Retention time units | Minutes |