Metadata details for analysis AN004933 | |
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Study ID | ST003003 |
Analysis ID | AN004933 |
Study Title | Gut microbiota and metabolites in estrus cycle and their changes in a menopausal transition rat model with typical neuroendocrine aging |
Institute | Fudan University |
Species | Rattus norvegicus |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
Chromatography Type | Reversed phase |
Chromatography Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
Solvent A | 100% water; 0.1%formic acid |
Solvent B | 100% acetonitrile; 0.1%formic acid |
Gradient | 0.35 mL/min: 5-20% B, 0-2 min; 20-25% B, 2-4 min; 25-60% B, 4-9 min; 60-100% B, 9-14 min; 100% B, 14-16 min; 100-5% B, 16-16.1 min; 5% B, 16.1-18.1 min |
Flow rate | 0.35 mL/min |
Column Temperature | 50 |
Retention time units | Minutes |