| Metadata details for analysis AN004934 | |
|---|---|
| Study ID | ST003003 |
| Analysis ID | AN004934 |
| Study Title | Gut microbiota and metabolites in estrus cycle and their changes in a menopausal transition rat model with typical neuroendocrine aging |
| Institute | Fudan University |
| Species | Rattus norvegicus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Solvent A | 100% water; 0.1%formic acid |
| Solvent B | 100% acetonitrile; 0.1%formic acid |
| Gradient | 0.35 mL/min: 5-20% B, 0-2 min; 20-25% B, 2-4 min; 25-60% B, 4-9 min; 60-100% B, 9-14 min; 100% B, 14-16 min; 100-5% B, 16-16.1 min; 5% B, 16.1-18.1 min |
| Flow rate | 0.35 mL/min |
| Column Temperature | 50 |
| Retention time units | Minutes |
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