| Metadata details for analysis AN004935 | |
|---|---|
| Study ID | ST003004 |
| Analysis ID | AN004935 |
| Study Title | Extracellular fluid metabolomics of BAT and eWAT |
| Institute | Harvard Medical School |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo orbitrap exploris 240 |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| Solvent A | 100% water; 25mM ammonium acetate; 25mM ammonium hydroxide |
| Solvent B | 100% acetonitrile |
| Gradient | The linear gradient eluted from 95% B (0.0–1 min), 95% B to 65% B (1–7.0 min), 65% B to 40% B (7.0–8.0 min), 40% B (8.0–9.0 min), 40% B to 95% B (9.0–9.1 min), then stayed at 95% B for 5.9 min. |
| Flow rate | 0.4 mL/min |
| Column Temperature | 25 °C |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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