Metadata details for analysis AN004937
Study IDST003006
Analysis IDAN004937
Study TitleBAT metabolomics from cold and TN mouse models
InstituteHarvard Medical School
SpeciesMus musculus
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo orbitrap exploris 240
MS Instrument TypeOrbitrap
Chromatography Instrument NameVanquish Horizon
Chromatography TypeHILIC
Chromatography ColumnWaters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Solvent A100% water; 25mM ammonium acetate; 25mM ammonium hydroxide
Solvent B100% acetonitrile
GradientThe linear gradient eluted from 95% B (0.0–1 min), 95% B to 65% B (1–7.0 min), 65% B to 40% B (7.0–8.0 min), 40% B (8.0–9.0 min), 40% B to 95% B (9.0–9.1 min), then stayed at 95% B for 5.9 min.
Flow rate0.4 mL/min
Column Temperature25 °C
Retention time unitsMinutes
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