Metadata details for analysis AN004950 | |
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Study ID | ST003017 |
Analysis ID | AN004950 |
Study Title | Lipidomics and plasma hormone reveal indicators of reproductive status in Florida manatees (Trichechus manatus latirostris) |
Institute | University of Florida |
Species | Florida manatee (Trichechus manatus latirostris) |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Thermo Vanquish |
Chromatography Type | Reversed phase |
Chromatography Column | Waters ACQUITY UPLC BEH C18(100 x 2.1mm,1.7um) |
Solvent A | 60:40 acetonitrile/water (v/v), 5mM ammonium formate and 0.1% formic acid |
Solvent B | 90:10 isopropanol/acetonitrile (v/v), 5mM ammonium formate and 0.1% formic acid |
Gradient | a linear increase of solvent B from 40% to 55% over 7 min, then increased to 65% by min 8, held at 65% for 4 min, linear increase to 95% over 8 min, increased to 100% over 2 min, solvent B was held at 100% for 5 min before decreasing to 40% in a tenth of a min. Finally, solvent B was held at 100% for another 3 min. |
Flow rate | 0.25 mL/min |
Column Temperature | 45 |
Retention time units | Seconds |