| Metadata details for analysis AN004967 | |
|---|---|
| Study ID | ST003030 |
| Analysis ID | AN004967 |
| Study Title | A small molecule macrophage migration inhibitory factor agonist ameliorates age-related myocardial intolerance to ischemia-reperfusion insults via metabolic regulation |
| Institute | University of Mississippi Medical Center |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Phenomenex Synergi Hydro-RP (100 x 2mm,2.5um) |
| Solvent A | 97:3 LCMS grade water : methanol, 11 mM tributylamine, 15 mM acetic acid. |
| Solvent B | LCMS grade methanol |
| Gradient | 0 to 5 min 0% B, from 5 to 13 min 20% B, from 13 to 15.5 min 55% B, from 15.5 to 19 min 95% B, and from 19 to 25 min 0% B |
| Flow rate | 200 µL/min |
| Column Temperature | 25 |
| Retention time units | Seconds |
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