Metadata details for analysis AN004967 | |
---|---|
Study ID | ST003030 |
Analysis ID | AN004967 |
Study Title | A small molecule macrophage migration inhibitory factor agonist ameliorates age-related myocardial intolerance to ischemia-reperfusion insults via metabolic regulation |
Institute | University of Mississippi Medical Center |
Species | Mus musculus |
Ion_mode | NEGATIVE |
MS type | ESI |
MS Instrument Name | Thermo Exactive Plus Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
Chromatography Type | Reversed phase |
Chromatography Column | Phenomenex Synergi Hydro-RP (100 x 2mm,2.5um) |
Solvent A | 97:3 LCMS grade water : methanol, 11 mM tributylamine, 15 mM acetic acid. |
Solvent B | LCMS grade methanol |
Gradient | 0 to 5 min 0% B, from 5 to 13 min 20% B, from 13 to 15.5 min 55% B, from 15.5 to 19 min 95% B, and from 19 to 25 min 0% B |
Flow rate | 200 µL/min |
Column Temperature | 25 |
Retention time units | Seconds |