Metabolomics Workbench : NIH Data Repository

Metadata details for analysis AN005004
Study ID	ST003052
Analysis ID	AN005004
Study Title	Synaptic vesicle lipidomics captures signatures of aging and synucleinopathy
Institute	Weill Cornell Medicine
Species	Mus musculus
Ion_mode	POSITIVE
MS type	ESI
MS Instrument Name	Thermo Q Exactive HF hybrid Orbitrap
MS Instrument Type	Orbitrap
Chromatography Instrument Name	Thermo Vanquish
Chromatography Type	Reversed phase
Chromatography Column	Thermo Accucore C30 (150 x 2.1mm,2.6um)
Solvent A	60% ACN, 40% H2O, 10 mM ammonium formate with 0.1% formic acid
Solvent B	90% IPA, 10% ACN, 10 mM ammonium formate with 0.1% formic acid
Gradient	0-4 min: 32% B; 4-5 min: 45% B; 5-8 min: 52% B; 8-11 min: 66% B; 11-14 min: 66% B; 14-18 min: 70% B, 18-21 min: 75% B; 21-25.01 min, 97% B; 25.01-30 min: 32% B
Flow rate	0.26 ml/min
Column Temperature	55
Retention time units	Minutes
Study ID	ST003052
Analysis ID	AN005004
Study Title	Synaptic vesicle lipidomics captures signatures of aging and synucleinopathy
Institute	Weill Cornell Medicine
Species	Mus musculus
Ion_mode	POSITIVE
MS type	ESI
MS Instrument Name	Thermo Q Exactive HF hybrid Orbitrap
MS Instrument Type	Orbitrap
Chromatography Instrument Name	Thermo Vanquish
Chromatography Type	Reversed phase
Chromatography Column	Thermo Accucore C30 (150 x 2.1mm,2.6um)
Solvent A	60% ACN, 40% H2O, 10 mM ammonium formate with 0.1% formic acid
Solvent B	90% IPA, 10% ACN, 10 mM ammonium formate with 0.1% formic acid
Gradient	0-4 min: 32% B; 4-5 min: 45% B; 5-8 min: 52% B; 8-11 min: 66% B; 11-14 min: 66% B; 14-18 min: 70% B, 18-21 min: 75% B; 21-25.01 min, 97% B; 25.01-30 min: 32% B
Flow rate	0.26 ml/min
Column Temperature	55
Retention time units	Minutes