Metadata details for analysis AN005075
Study IDST003101
Analysis IDAN005075
Study TitleParallel pheromonal, metabolite, and lipid analyses reveal patterns associated with early life transitions and ovary activation in honey bee (Apis mellifera) queens
InstituteLife Sciences Institute, The University of British Columbia
SpeciesApis mellifera
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameBruker Impact II
MS Instrument TypeQTOF
Chromatography Instrument NameThermo Vanquish
Chromatography TypeReversed phase
Chromatography ColumnWaters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Solvent A100% water; 10 mM ammonium formate; 0.1% formic acid
Solvent B10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid
Gradient0 min (20% B), 0–2 min (20% B), 2–11 min (80% B), 11–11.5 min (99% B), 11.5–13.2 min (99% B), 13.2–14 min (5% B), and 14–18 min (5% B).
Flow rate0.4 ml/min
Column Temperature65
Retention time unitsMinutes
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