| Metadata details for analysis AN005113 | |
|---|---|
| Study ID | ST003119 |
| Analysis ID | AN005113 |
| Study Title | Bleach protects algal crops from novel pathogen but increases stress tolerance traits and virulence factors within the associated bacterial microbiome |
| Institute | National Renewable Energy Lab (NREL) |
| Species | |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Solvent A | 97% 18.2 mΩ-cm water/3% acetonitrile; 20 mM ammonium acetate; 15 mM ammonium hydroxide |
| Solvent B | 95% acetonitrile/5% 18.2 mΩ-cm water; 20 mM ammonium acetate; 15 mM ammonium hydroxide |
| Gradient | 90% solvent B for 1 min followed by a linear gradient down to 75% solvent B for 23 min, a linear gradient down to 45% solvent B in 2 min, a linear gradient down to 25% B in 4 min, followed by a step to the starting composition of 90% B and hold for 6 min for column regeneration for a total run time of 36 min. |
| Flow rate | 0.2 mL/min |
| Column Temperature | 25°C |
| Retention time units | Minutes |
| Study ID | ST003119 |
| Analysis ID | AN005113 |
| Study Title | Bleach protects algal crops from novel pathogen but increases stress tolerance traits and virulence factors within the associated bacterial microbiome |
| Institute | National Renewable Energy Lab (NREL) |
| Species | |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Solvent A | 97% 18.2 mΩ-cm water/3% acetonitrile; 20 mM ammonium acetate; 15 mM ammonium hydroxide |
| Solvent B | 95% acetonitrile/5% 18.2 mΩ-cm water; 20 mM ammonium acetate; 15 mM ammonium hydroxide |
| Gradient | 90% solvent B for 1 min followed by a linear gradient down to 75% solvent B for 23 min, a linear gradient down to 45% solvent B in 2 min, a linear gradient down to 25% B in 4 min, followed by a step to the starting composition of 90% B and hold for 6 min for column regeneration for a total run time of 36 min. |
| Flow rate | 0.2 mL/min |
| Column Temperature | 25°C |
| Retention time units | Minutes |
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