| Metadata details for analysis AN005120 | |
|---|---|
| Study ID | ST003124 |
| Analysis ID | AN005120 |
| Study Title | Serum metabolites in inherited retinal degenerations |
| Institute | National Taiwan University |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| Solvent A | Acetonitrile/water (4/6); 5 mM ammonium acetate |
| Solvent B | Isopropyl alcohol/acetonitrile (9/1); 5 mM ammonium acetate |
| Gradient | 0–0.5 min, 15% B; 0.5–4 min, 15–70% B; 4–15 min, 70–99% B; 15–16.5 min, 99% B; 16.15–17 min, 99–15% B; and 17–19 min, 15% B |
| Flow rate | 0.2 mL/min |
| Column Temperature | 50℃ |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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