| Metadata details for analysis AN005146 | |
|---|---|
| Study ID | ST003135 |
| Analysis ID | AN005146 |
| Study Title | Metabolomic study of T effector and T regulatory cells in severe allergic patients |
| Institute | Universidad San Pablo-CEU, CEU Universities |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6550 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 1290 Infinity II |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) |
| Solvent A | 100% water; 0.1% of formic acid |
| Solvent B | 100% acetonitrile; 0.1% of formic acid |
| Gradient | 0-1 min: 5% B; 1-8 min: linear increase until 80% B; 8-12.5 min: linear increase until 100% B. Then the equipment returned to the initial conditions in 0.5 min, which were held for 3 min for column reconditioning. |
| Flow rate | 0.6 mL/min |
| Column Temperature | 60 ÂșC |
| Retention time units | Minutes |