Metadata details for analysis AN005156 | |
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Study ID | ST003142 |
Analysis ID | AN005156 |
Study Title | Effect of liver Acox1 knockout on serum lipidome in mice |
Institute | Washington University in St. Louis |
Species | Mus musculus |
Ion_mode | NEGATIVE |
MS type | ESI |
MS Instrument Name | Bruker Impact HD |
MS Instrument Type | QTOF |
Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
Chromatography Type | Reversed phase |
Chromatography Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
Solvent A | 50% Methanol/40% Acetonitrile/10% Water; 10 mM Ammonium formate |
Solvent B | 95% Isopropyl alcohol/5% water; 10 mM Ammonium formate |
Gradient | t = 0 min, 5% B; t = 10 min, 40% B; t = 18.8 min, 98% B; t = 20.5 min, 98% B. |
Flow rate | 250 μL/min. |
Column Temperature | 42 °C |
Retention time units | Minutes |