| Metadata details for analysis AN005156 | |
|---|---|
| Study ID | ST003142 |
| Analysis ID | AN005156 |
| Study Title | Effect of liver Acox1 knockout on serum lipidome in mice |
| Institute | Washington University in St. Louis |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Bruker Impact HD |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| Solvent A | 50% Methanol/40% Acetonitrile/10% Water; 10 mM Ammonium formate |
| Solvent B | 95% Isopropyl alcohol/5% water; 10 mM Ammonium formate |
| Gradient | t = 0 min, 5% B; t = 10 min, 40% B; t = 18.8 min, 98% B; t = 20.5 min, 98% B. |
| Flow rate | 250 μL/min. |
| Column Temperature | 42 °C |
| Retention time units | Minutes |
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