| Metadata details for analysis AN005199 | |
|---|---|
| Study ID | ST003168 |
| Analysis ID | AN005199 |
| Study Title | Lipidomic analysis of cryopreserved human cardiac tissue from young and ageing adults |
| Institute | University of Sydney |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF-X Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters HPLC C18 (100 x 2.1mm, 1.7um) |
| Solvent A | 60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
| Gradient | 0 min, 80:20 A/B; 3 min, 80:20 A/B; 5.5 min, 55:45 A/B; 8 min, 35:65 A/B; 13 min, 15:85 A/B; 14 min, 0:100 A/B; 20 min, 0:100 A/B; 20.2 min, 70:30 A/B; 27 min, 70:30 A/B |
| Flow rate | 0.28 mL/min |
| Column Temperature | 32 |
| Retention time units | Minutes |
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