Metadata details for analysis AN005199
Study IDST003168
Analysis IDAN005199
Study TitleLipidomic analysis of cryopreserved human cardiac tissue from young and ageing adults
InstituteUniversity of Sydney
SpeciesHomo sapiens
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF-X Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Vanquish
Chromatography TypeReversed phase
Chromatography ColumnWaters HPLC C18 (100 x 2.1mm, 1.7um)
Solvent A60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid
Solvent B90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid
Gradient0 min, 80:20 A/B; 3 min, 80:20 A/B; 5.5 min, 55:45 A/B; 8 min, 35:65 A/B; 13 min, 15:85 A/B; 14 min, 0:100 A/B; 20 min, 0:100 A/B; 20.2 min, 70:30 A/B; 27 min, 70:30 A/B
Flow rate0.28 mL/min
Column Temperature32
Retention time unitsMinutes
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