Metadata details for analysis AN005235 | |
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Study ID | ST003187 |
Analysis ID | AN005235 |
Study Title | Untargeted plasma metabolomics on bacterial culture supernatants |
Institute | Harvard University |
Species | Escherichia coli |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Agilent 6530 QTOF |
MS Instrument Type | QTOF |
Chromatography Instrument Name | Agilent 1260 Infinity HPLC |
Chromatography Type | HILIC |
Chromatography Column | Waters Atlantis HILIC (150 x 2.1mm,3um) |
Solvent A | 100% water; 0.1% formic acid |
Solvent B | 100% acetonitrile; 0.1% formic acid |
Gradient | 0.5 minute at 5% mobile phase B followed by a linear gradient to 40% mobile phase B over 10 minutes and then maintained isocratically for 4.5 minutes before returning to 5% mobile phase B by gradient over 2 minutes |
Flow rate | 250 μL/min |
Column Temperature | 30 |
Retention time units | Minutes |