Metadata details for analysis AN005235
Study IDST003187
Analysis IDAN005235
Study TitleUntargeted plasma metabolomics on bacterial culture supernatants
InstituteHarvard University
SpeciesEscherichia coli
Ion_modePOSITIVE
MS typeESI
MS Instrument NameAgilent 6530 QTOF
MS Instrument TypeQTOF
Chromatography Instrument NameAgilent 1260 Infinity HPLC
Chromatography TypeHILIC
Chromatography ColumnWaters Atlantis HILIC (150 x 2.1mm,3um)
Solvent A100% water; 0.1% formic acid
Solvent B100% acetonitrile; 0.1% formic acid
Gradient0.5 minute at 5% mobile phase B followed by a linear gradient to 40% mobile phase B over 10 minutes and then maintained isocratically for 4.5 minutes before returning to 5% mobile phase B by gradient over 2 minutes
Flow rate250 μL/min
Column Temperature30
Retention time unitsMinutes
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