| Metadata details for analysis AN005246 | |
|---|---|
| Study ID | ST003196 |
| Analysis ID | AN005246 |
| Study Title | Unveiling cellular changes in leukaemia cell line HL-60 after cannabidiol treatment through lipidomics |
| Institute | Universidad CEU- San Pablo |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6545 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 1290 Infinity II |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| Solvent A | 90% Water, 10% Methanol; 10 mM ammonium acetate, 0.2 mM ammonium fluoride |
| Solvent B | 20% acetonitrile, 30% Methanol, 50% Isopropanol; 10 mM ammonium acetate, 0.2 mM ammonium fluoride |
| Gradient | Started at 70% of B at 0-1 min, 86% B at 3.5-10 min, 100% B at 11-17 min. The starting conditions were recovered by minute 17. |
| Flow rate | 0.6 mL/min |
| Column Temperature | 50ºC |
| Retention time units | Minutes |
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