Metadata details for analysis AN005262
Study IDST003210
Analysis IDAN005262
Study TitleUntargeted metabolomics analysis on single cells and cell clusters of TNBC cell lines using mass spectrometry
InstituteNanjing Medical University
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF hybrid Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Dionex Ultimate 3000
Chromatography TypeHILIC
Chromatography ColumnAgilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm, 2.7um)
Solvent A100% Water; 25 mM Ammonium acetate; 25 mM Ammonium hydroxide
Solvent B100% Acetonitrile
Gradient85% B (0 min) to 85% B (1 min) to 65% B (12 min) to 40% B (12.1 min) to 40% B (15 min) to 85% B (15.1 min) to 85% B (20 min), the flow gradient increases linearly between the time points mentioned.
Flow rate0.3 mL/min
Column Temperature40
Retention time unitsMinutes
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