| Metadata details for analysis AN005263 | |
|---|---|
| Study ID | ST003210 |
| Analysis ID | AN005263 |
| Study Title | Untargeted metabolomics analysis on single cells and cell clusters of TNBC cell lines using mass spectrometry |
| Institute | Nanjing Medical University |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | HILIC |
| Chromatography Column | Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm, 2.7um) |
| Solvent A | 100% Water; 25 mM Ammonium acetate; 25 mM Ammonium hydroxide |
| Solvent B | 100% Acetonitrile |
| Gradient | 85% B (0 min) to 85% B (1 min) to 65% B (12 min) to 40% B (12.1 min) to 40% B (15 min) to 85% B (15.1 min) to 85% B (20 min), the flow gradient increases linearly between the time points mentioned. |
| Flow rate | 0.3 mL/min |
| Column Temperature | 40 |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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