Metadata details for analysis AN005264
Study IDST003210
Analysis IDAN005264
Study TitleUntargeted metabolomics analysis on single cells and cell clusters of TNBC cell lines using mass spectrometry
InstituteNanjing Medical University
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF hybrid Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Dionex Ultimate 3000
Chromatography TypeReversed phase
Chromatography ColumnPhenomenex Kinetex C18 (100 x 2.1mm,2.6um)
Solvent A100% Water; 0.1% Formic acid
Solvent B100% Acetonitrile; 0.1% Formic acid
Gradient10% B (0 min) to 30% B (1 min) to 95% B (19 min) to 95% B (20 min) to 10% B (20.1 min) to 10% B (23 min), the flow gradient increases linearly between the time points mentioned.
Flow rate0.4 mL/min
Column Temperature40
Retention time unitsMinutes
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