| Metadata details for analysis AN005265 | |
|---|---|
| Study ID | ST003210 |
| Analysis ID | AN005265 |
| Study Title | Untargeted metabolomics analysis on single cells and cell clusters of TNBC cell lines using mass spectrometry |
| Institute | Nanjing Medical University |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Phenomenex Kinetex C18 (100 x 2.1mm,2.6um) |
| Solvent A | 100% Water; 0.1% Formic acid |
| Solvent B | 100% Acetonitrile; 0.1% Formic acid |
| Gradient | 10% B (0 min) to 30% B (1 min) to 95% B (19 min) to 95% B (20 min) to 10% B (20.1 min) to 10% B (23 min), the flow gradient increases linearly between the time points mentioned. |
| Flow rate | 0.4 mL/min |
| Column Temperature | 40 |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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