| Metadata details for analysis AN005275 | |
|---|---|
| Study ID | ST003217 |
| Analysis ID | AN005275 |
| Study Title | Measuring UQ/UQH2 in WT and RquA 143B cell in Hypoxia |
| Institute | UMass Chan Medical School |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Phenomenex Luna PFP(2) (100 x 2mm, 3um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | 0 - 3 min, 30% A; 3 - 3.25 min, 30% - 2% A; 3.25 - 5min, 2% A; 5 - 6min, 2% - 1% A; 6 - 8.75min, 1% A; 8.75 - 9min, 1% - 30% A; 9 - 10min, 30% A |
| Flow rate | 0.5mL/min |
| Column Temperature | 25 |
| Retention time units | Minutes |
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