Metabolomics Workbench : NIH Data Repository

Metadata details for analysis AN005284
Study ID	ST003223
Analysis ID	AN005284
Study Title	EVALUATION OF THE INFLUENCE OF ENVIRONMENTAL CONDITIONS ON THE METABOLOME OF Psychotria viridis LEAVES Ruiz & Pav.: Lipidomics approach
Institute	University of Campinas
Species	Psychotria viridis
Ion_mode	POSITIVE
MS type	ESI
MS Instrument Name	Thermo Q Exactive Orbitrap
MS Instrument Type	Orbitrap
Chromatography Instrument Name	Thermo UltiMate 3000 RSLCnano
Chromatography Type	Reversed phase
Chromatography Column	Merck Supelco Titan C18 (100 × 2.1 mm, 1.9 µm)
Solvent A	60% Water, 40% Acetonitrile; 10 mM ammonium acetate
Solvent B	90% Isopropanol, 10% Acetonitrile; 10 mM ammonium acetate
Gradient	0.20 mL/min: 0-2 min, 40% B; 2-3 min, 50% B; 3-6 min, 50% B; 6.1-8 min, 70% B; 8-9 min, 100%B; 9-11 mim, 100%; 11-12 min, 40%B; 12-14 min; 40% B.
Flow rate	0.20 mL/min
Column Temperature	40
Retention time units	Minutes
Study ID	ST003223
Analysis ID	AN005284
Study Title	EVALUATION OF THE INFLUENCE OF ENVIRONMENTAL CONDITIONS ON THE METABOLOME OF Psychotria viridis LEAVES Ruiz & Pav.: Lipidomics approach
Institute	University of Campinas
Species	Psychotria viridis
Ion_mode	POSITIVE
MS type	ESI
MS Instrument Name	Thermo Q Exactive Orbitrap
MS Instrument Type	Orbitrap
Chromatography Instrument Name	Thermo UltiMate 3000 RSLCnano
Chromatography Type	Reversed phase
Chromatography Column	Merck Supelco Titan C18 (100 × 2.1 mm, 1.9 µm)
Solvent A	60% Water, 40% Acetonitrile; 10 mM ammonium acetate
Solvent B	90% Isopropanol, 10% Acetonitrile; 10 mM ammonium acetate
Gradient	0.20 mL/min: 0-2 min, 40% B; 2-3 min, 50% B; 3-6 min, 50% B; 6.1-8 min, 70% B; 8-9 min, 100%B; 9-11 mim, 100%; 11-12 min, 40%B; 12-14 min; 40% B.
Flow rate	0.20 mL/min
Column Temperature	40
Retention time units	Minutes