| Metadata details for analysis AN005420 | |
|---|---|
| Study ID | ST003308 |
| Analysis ID | AN005420 |
| Study Title | An untargeted metabolic profiling strategy for the dissection of the oat (Avena sativa L.) plant innate immune response to various Pseudomonas syringae pathovars |
| Institute | University of Johannesburg |
| Species | Avena sativa L. |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Waters Synapt G1 |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Waters Acquity |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Solvent A | 97.5% MilliQ water/2.5% isopropanol; 0.1% formic acid |
| Solvent B | 97,5% acetonitrile/2.5% isopropanol; 0.1% formic acid |
| Gradient | The initial conditions were 95% A and 5% B and held for 1 min. A gradient was applied to change the chromatographic conditions to 10% A and 90% B at 25 min; and changed to 5% A and 95% B at 25.10 min. These conditions were held for 2 min and then changed to the initial conditions at 28 min. The analytical column was allowed to equilibrate for 2 min before each subsequent injection. |
| Flow rate | 0.4 mL/min |
| Column Temperature | 60 |
| Retention time units | Minutes |
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