Metabolomics Workbench : NIH Data Repository : Analyze Studies

Data for ST002125

(Analysis AN003478): Average values per metabolite and experimental factor (Units:Peak Area)

Metabolite structure	F1	F2	F3	F4	F5	F6	F7	F8	F9	F10	F11	F12	F13	F14
[13C, 15N]Alanine, 2TBDMS	-	-	67833.33	6783333.33	6163333.33	5603333.33	67500.00	6415000.00	6163333.33	5575000.00	6663333.33	4943333.33	5763333.33	61633.33
[13C, 15N]Aspartic acid, 3TBDMS	-	-	112433.33	11243333.33	4600000.00	6190000.00	114666.67	10010000.00	4430000.00	5350000.00	8903333.33	3426666.67	5426666.67	78666.67
[13C, 15N]Glutamic acid, 3TBDMS	-	-	72833.33	7283333.33	2296666.67	3880000.00	69400.00	6030000.00	1710000.00	3195000.00	2766666.67	561666.67	1633333.33	32966.67
[13C, 15N]Glycine, 2TBDMS	-	-	78533.33	6770000.00	4310000.00	5260000.00	76833.33	6602500.00	4653333.33	4965000.00	5670000.00	2823333.33	4050000.00	57933.33
[13C, 15N]Histidine, 3TBDMS	-	-	49333.33	4933333.33	3606666.67	4146666.67	47933.33	4660000.00	3296666.67	3930000.00	3346666.67	969666.67	2303333.33	40200.00
[13C, 15N]Isoleucine, 2TBDMS	-	-	169666.67	16966666.67	10350000.00	14466666.67	163000.00	16025000.00	12533333.33	14350000.00	11383333.33	3540000.00	6590000.00	132666.67
[13C, 15N]Leucine, 2TBDMS	-	-	199666.67	19966666.67	8636666.67	13900000.00	222000.00	19650000.00	7853333.33	13750000.00	12700000.00	4363333.33	8346666.67	134666.67
[13C, 15N]Lysine, 3TBDMS	-	-	79200.00	7920000.00	3886666.67	5540000.00	83533.33	7655000.00	3863333.33	4945000.00	6563333.33	3200000.00	5380000.00	55300.00
[13C, 15N]Methionine, 2TBDMS	-	-	183000.00	18300000.00	12266666.67	14666666.67	160333.33	15625000.00	11100000.00	12600000.00	12653333.33	7136666.67	8550000.00	141333.33
[13C, 15N]Phenylalanine, 2TBDMS	-	-	133000.00	13300000.00	6236666.67	9693333.33	136000.00	12612500.00	6963333.33	10800000.00	12833333.33	4926666.67	8153333.33	115000.00
[13C, 15N]Proline, 2TBDMS	-	-	136000.00	13600000.00	8343333.33	10900000.00	155666.67	14975000.00	7670000.00	12000000.00	7966666.67	2553333.33	5506666.67	84433.33
[13C, 15N]Serine, 3TBDMS	-	-	129000.00	12900000.00	8016666.67	10153333.33	138333.33	13250000.00	7796666.67	10850000.00	9266666.67	6610000.00	7816666.67	79800.00
[13C, 15N]Threonine, 3TBDMS	-	-	18733.33	1873333.33	1083333.33	1493333.33	19266.67	1832500.00	1153333.33	1605000.00	1773333.33	856333.33	1163333.33	13733.33
[13C, 15N]Tyrosine, 3TBDMS	-	-	833000.00	83300000.00	39500000.00	61766666.67	849666.67	78325000.00	32066666.67	60900000.00	48633333.33	21833333.33	32666666.67	507666.67
[13C, 15N]Valine, 2TBDMS	-	-	62933.33	62933333.33	43166666.67	54266666.67	70833.33	63925000.00	39166666.67	52300000.00	46100000.00	15566666.67	28300000.00	41900.00
[13C]Alpha_Ketoglutarate	-	-	6246666.67	18133.33	20733.33	20466.67	6213333.33	15300.00	18333.33	17200.00	27633.33	76900.00	44033.33	4103333.33
[13C]Citrate	-	-	14600000.00	31733.33	45666.67	37666.67	16233333.33	33900.00	41166.67	36250.00	57766.67	199333.33	116000.00	8053333.33
[13C]Fumatate	-	-	62266666.67	122333.33	145000.00	129000.00	65133333.33	140500.00	157000.00	157500.00	205333.33	305333.33	254333.33	41066666.67
[13C]Malate	-	-	22833333.33	65300.00	76133.33	73500.00	23600000.00	68075.00	76100.00	71250.00	86500.00	121333.33	93666.67	10453333.33
[13C]Succinate	-	-	64633333.33	104200.00	131333.33	120000.00	64566666.67	103425.00	139333.33	122500.00	159333.33	268000.00	211333.33	41833333.33
Alanine, 2TBDMS	318333333.33	78000000.00	5043333.33	-	-	-	5400000.00	-	-	-	-	-	-	1413333.33
Alpha_Ketoglutarate	-	-	6246666.67	-	-	-	6213333.33	-	-	-	-	-	-	4103333.33
Aspartic acid, 3TBDMS	3186666666.67	837666666.67	142000000.00	-	-	-	143333333.33	-	-	-	-	-	-	44966666.67
Citrate	-	-	14600000.00	-	-	-	16233333.33	-	-	-	-	-	-	8053333.33
Fumatate	-	-	62266666.67	-	-	-	65133333.33	-	-	-	-	-	-	41066666.67
Glutamic acid, 3TBDMS	3780000000.00	1743333333.33	149333333.33	-	-	-	172333333.33	-	-	-	-	-	-	42000000.00
Glutamine, 3TBDMS	365666666.67	151666666.67	1706666.67	-	-	-	1576666.67	-	-	-	-	-	-	410000.00
Glycine, 2TBDMS	13866666666.67	3090000000.00	580000000.00	-	-	-	470666666.67	-	-	-	-	-	-	240000000.00
Histidine, 3TBDMS	221666666.67	76733333.33	274000.00	-	-	-	248666.67	-	-	-	-	-	-	48966.67
Hydroxyproline, (E)-, 3TBDMS	97566666.67	40000000.00	5100000.00	-	-	-	5006666.67	-	-	-	-	-	-	1273333.33
Isoleucine, 2TBDMS	2120000000.00	433333333.33	17066666.67	-	-	-	16800000.00	-	-	-	-	-	-	5290000.00
L-Cysteine, 3TBDMS	14500000.00	2883333.33	3063333.33	-	-	-	3250000.00	-	-	-	-	-	-	771333.33
Leucine, 2TBDMS	1090000000.00	611666666.67	43866666.67	-	-	-	41700000.00	-	-	-	-	-	-	13333333.33
Lysine, 3TBDMS	2143333333.33	472333333.33	16066666.67	-	-	-	13533333.33	-	-	-	-	-	-	3940000.00
Malate	-	-	22833333.33	-	-	-	23600000.00	-	-	-	-	-	-	10453333.33
Methionine, 2TBDMS	1196666666.67	296666666.67	13300000.00	-	-	-	14466666.67	-	-	-	-	-	-	3330000.00
Phenylalanine, 2TBDMS	2276666666.67	424333333.33	5720000.00	-	-	-	6930000.00	-	-	-	-	-	-	2020000.00
Proline, 2TBDMS	1536666666.67	418666666.67	70233333.33	-	-	-	75000000.00	-	-	-	-	-	-	11966666.67
Serine, 3TBDMS	3256666666.67	839666666.67	53300000.00	-	-	-	59966666.67	-	-	-	-	-	-	20433333.33
Succinate	-	-	64633333.33	-	-	-	64566666.67	-	-	-	-	-	-	41833333.33
Threonine, 3TBDMS	328000000.00	97066666.67	7396666.67	-	-	-	6806666.67	-	-	-	-	-	-	2506666.67
Tyrosine, 3TBDMS	4636666666.67	1566666666.67	20333333.33	-	-	-	23433333.33	-	-	-	-	-	-	5626666.67
Valine, 2TBDMS	3323333333.33	890333333.33	48533333.33	-	-	-	46966666.67	-	-	-	-	-	-	12866666.67

Factors:

F1	Source_Name[gating]:Bone marrow supernatant \| Treatment:5 FU 2 weeks \| Batch:1c \| Note:Unlabeled AAs were used for total AA (FigS2.D)
F2	Source_Name[gating]:Bone marrow supernatant \| Treatment:HSC Basal \| Batch:1c \| Note:Unlabeled AAs were used for total AA (FigS2.D)
F3	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 1 h \| Batch:1c \| Note:[13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J).
F4	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h \| Batch:1b \| Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F5	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h \| Batch:1b \| Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F6	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h \| Batch:1b \| Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F7	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 1 h \| Batch:1c \| Note:[13C, 15N] AAs were used for AA uptake (Fig2.B); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C) and TCA substrate levels (Fig2.K).
F8	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h \| Batch:1b \| Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F9	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h \| Batch:1b \| Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F10	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h \| Batch:1b \| Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F11	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h \| Batch:1b \| Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D).
F12	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h \| Batch:1b \| Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D).
F13	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h \| Batch:1b \| Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D).
F14	Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) \| Treatment:HSC Basal or Ctrl, [13C, 15N] AAs were incorporated for 1 h \| Batch:1c \| Note:[13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J).