Data for ST002126   

(Analysis AN003479): Average values per metabolite and experimental factor (Units:Peak Area)

Metabolite structureAll dataF1F2F3F4F5F6F7F8F9F10
[13C, 15N]Alanine, 2TBDMS
67833.33 2790000.00 841666.67 1456666.67 61633.33 - 63666.67 3103333.33 161000.00 718333.33
[13C, 15N]Aspartic acid, 3TBDMS
112433.33 637333.33 313000.00 410000.00 78666.67 - 81833.33 584333.33 94200.00 208000.00
[13C, 15N]Glutamic acid, 3TBDMS
72833.33 535333.33 205000.00 363666.67 32966.67 - 66500.00 565000.00 37433.33 181000.00
[13C, 15N]Glycine, 2TBDMS
78533.33 7196666.67 3873333.33 4796666.67 57933.33 - 72466.67 7116666.67 1830000.00 3143333.33
[13C, 15N]Histidine, 3TBDMS
49333.33 447000.00 123000.00 255000.00 40200.00 - 45000.00 450000.00 25200.00 82233.33
[13C, 15N]Isoleucine, 2TBDMS
169666.67 2776666.67 1226666.67 1950000.00 132666.67 - 151666.67 2850000.00 239000.00 699666.67
[13C, 15N]Leucine, 2TBDMS
199666.67 2663333.33 873000.00 1736666.67 134666.67 - 186000.00 3193333.33 226000.00 898333.33
[13C, 15N]Lysine, 3TBDMS
79200.00 1223333.33 471333.33 775666.67 55300.00 - 97666.67 1143333.33 41300.00 279666.67
[13C, 15N]Methionine, 2TBDMS
183000.00 1643333.33 504666.67 891333.33 141333.33 - 159000.00 1490000.00 87900.00 435666.67
[13C, 15N]Phenylalanine, 2TBDMS
133000.00 2173333.33 1022666.67 1250000.00 115000.00 - 143666.67 2403333.33 248666.67 576666.67
[13C, 15N]Proline, 2TBDMS
136000.00 1550000.00 442666.67 741333.33 84433.33 - 156666.67 1566666.67 111333.33 396000.00
[13C, 15N]Serine, 3TBDMS
129000.00 3020000.00 815000.00 2026666.67 79800.00 - 165666.67 2990000.00 157000.00 777666.67
[13C, 15N]Threonine, 3TBDMS
18733.33 688000.00 278333.33 398666.67 13733.33 - 21966.67 686333.33 78700.00 228333.33
[13C, 15N]Tyrosine, 3TBDMS
833000.00 6770000.00 1666666.67 3076666.67 507666.67 - 838000.00 6380000.00 388333.33 1553333.33
[13C, 15N]Valine, 2TBDMS
62933.33 6710000.00 2816666.67 4320000.00 41900.00 - 63000.00 7063333.33 786000.00 2110000.00
[13C]Alpha_Ketoglutarate
6246666.67 4913.33 7403.33 6453.33 4103333.33 - - 6803.33 19466.67 10240.00
[13C]Citrate
14600000.00 77833.33 99000.00 84700.00 8053333.33 - - 141333.33 260666.67 202000.00
[13C]Fumatate
62266666.67 94000.00 119333.33 118000.00 41066666.67 - - 143666.67 344333.33 250666.67
[13C]Malate
22833333.33 11896.67 14000.00 12866.67 10453333.33 - - 26666.67 43333.33 31300.00
[13C]Succinate
64633333.33 32733.33 51433.33 45600.00 41833333.33 - - 48566.67 119666.67 75366.67
Alanine, 2TBDMS
5043333.33 - - - 1413333.33 886000.00 2126666.67 - - -
Alpha_Ketoglutarate
6246666.67 - - - 4103333.33 3343333.33 3870000.00 - - -
Aspartic acid, 3TBDMS
142000000.00 - - - 44966666.67 20900000.00 36833333.33 - - -
Citrate
14600000.00 - - - 8053333.33 1473333.33 4043333.33 - - -
Fumatate
62266666.67 - - - 41066666.67 34600000.00 40500000.00 - - -
Glutamic acid, 3TBDMS
149333333.33 - - - 42000000.00 15100000.00 41700000.00 - - -
Glutamine, 3TBDMS
1706666.67 - - - 410000.00 239666.67 511333.33 - - -
Glycine, 2TBDMS
580000000.00 - - - 240000000.00 98133333.33 140666666.67 - - -
Histidine, 3TBDMS
274000.00 - - - 48966.67 26033.33 39266.67 - - -
Hydroxyproline, (E)-, 3TBDMS
5100000.00 - - - 1273333.33 715333.33 1536666.67 - - -
Isoleucine, 2TBDMS
17066666.67 - - - 5290000.00 2720000.00 4463333.33 - - -
L-Cysteine, 3TBDMS
3063333.33 - - - 771333.33 33266.67 70033.33 - - -
Leucine, 2TBDMS
43866666.67 - - - 13333333.33 5336666.67 9466666.67 - - -
Lysine, 3TBDMS
16066666.67 - - - 3940000.00 2380000.00 4270000.00 - - -
Malate
22833333.33 - - - 10453333.33 7876666.67 10966666.67 - - -
Methionine, 2TBDMS
13300000.00 - - - 3330000.00 1096666.67 2546666.67 - - -
Phenylalanine, 2TBDMS
5720000.00 - - - 2020000.00 1343333.33 2390000.00 - - -
Proline, 2TBDMS
70233333.33 - - - 11966666.67 5850000.00 17766666.67 - - -
Serine, 3TBDMS
53300000.00 - - - 20433333.33 7436666.67 16266666.67 - - -
Succinate
64633333.33 - - - 41833333.33 33400000.00 37233333.33 - - -
Threonine, 3TBDMS
7396666.67 - - - 2506666.67 1245000.00 2333333.33 - - -
Tyrosine, 3TBDMS
20333333.33 - - - 5626666.67 3200000.00 5703333.33 - - -
Valine, 2TBDMS
48533333.33 - - - 12866666.67 5783333.33 11996666.67 - - -

Factors:

F1Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J).
F2Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L).
F3Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L).
F4Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L).
F5Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal or Ctrl, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J).
F6Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal or Ctrl, NR-treatment 8 weeks, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig5J).
F7Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 8 weeks, 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig5J).
F8Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 8 weeks, 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L).
F9Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 8 weeks, 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L).
F10Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 8 weeks, 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L).
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