Clustering data with hclust algorithm for Determining the metabolic profile of wildtype and nos mutant Staphylococcus aureus grown in media lacking glucose using targeted LC/MS (Study ST000247)
ESI positive ion mode Organic Acids (Instrument:Thermo Scientific Quantiva) (Analysis AN000387)| Metabolite | Structure | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 | F13 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lactate | ME700675 | 0.93 | 0.07 | 0.11 | 0.07 | 0.05 | 0.09 | 0.09 | 11.18 | 0.09 | 0.05 | 0.04 | 0.13 | 0.10 |
| Pyruvate | ME700676 | 9.29 | 0.28 | 0.15 | 0.20 | 0.23 | 0.34 | 0.23 | 1.04 | 0.27 | 0.25 | 0.20 | 0.35 | 0.18 |
| Fumarate | ME700679 | 0.47 | 0.89 | 0.02 | 0.67 | 0.09 | 1.69 | 0.09 | 1.00 | NA | 5.38 | 0.53 | 1.14 | 0.04 |
| Malate | ME700680 | 2.61 | 0.32 | 0.13 | 0.35 | 0.35 | 0.63 | 0.41 | 1.96 | 0.03 | 3.47 | 1.78 | 0.43 | 0.21 |
| Succinate | ME700678 | 0.98 | 0.48 | 0.76 | 1.01 | 1.14 | 0.95 | 1.60 | 1.13 | 0.07 | 0.61 | 2.34 | 0.64 | 1.27 |
| a-KG | ME700681 | 0.50 | 2.28 | 0.07 | 0.55 | 0.03 | 3.04 | 0.08 | 0.65 | 0.07 | 1.24 | 0.07 | 3.72 | 0.08 |
| _3_HBA | ME700677 | 0.97 | 0.94 | NA | 1.05 | NA | 0.97 | NA | 1.04 | NA | 1.07 | NA | 1.00 | NA |
| Citrate | ME700682 | 2.58 | 1.16 | 0.01 | 1.44 | 0.00 | 1.84 | 0.01 | 2.48 | 0.01 | 1.19 | 0.01 | 1.93 | 0.01 |
Factors:
| F1 | Strain:N/A | Sample type:Media Only |
| F2 | Strain:nos complement | Sample type:Supernatant |
| F3 | Strain:nos complement | Sample type:Whole Cells |
| F4 | Strain:nos mutant | Sample type:Supernatant |
| F5 | Strain:nos mutant | Sample type:Whole Cells |
| F6 | Strain:nos SrrAB mutant nos complement | Sample type:Supernatant |
| F7 | Strain:nos SrrAB mutant nos complement | Sample type:Whole Cells |
| F8 | Strain:nos SrrAB mutant | Sample type:Supernatant |
| F9 | Strain:nos SrrAB mutant | Sample type:Whole Cells |
| F10 | Strain:SrrAB mutant | Sample type:Supernatant |
| F11 | Strain:SrrAB mutant | Sample type:Whole Cells |
| F12 | Strain:Wildtype | Sample type:Supernatant |
| F13 | Strain:Wildtype | Sample type:Whole Cells |
